Looks like the world's oldest known self-contained ecosystem was recently discovered:
Researchers in Israel have discovered eight previously unknown ancient animal species within 'a new and unique underground ecosystem' in central Israel, the Hebrew University of Jerusalem announced Tuesday.
The cave was completely sealed off from the world, including from water and nutrients seeping through rock crevices above. Scientists who discovered the cave believe it has been intact for millions of years.
All of the previously unknown organisms, including species of crustaceans and invertebrates, have no eyes. So over the past few million years they have evolved to have no eyes nor eye appedages. Are you listening creationists?